Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles

Fast and background-free three-dimensional (3D) live-cell imaging with lanthanide-doped upconverting nanoparticles.

We report on the development of a three-dimensional (3D) live-cell imaging technique with high spatiotemporal resolution using lanthanide-doped upconverting nanoparticles (UCNPs). It employs the sectioning capability of confocal microscopy except that the two-dimensional (2D) section images are acquired by wide-field epi-fluorescence microscopy. Although epi-fluorescence images are contaminated...

Live-cell imaging of drug delivery by mesoporous silica nanoparticles

Defining the Subcellular Interface of Nanoparticles by Live-Cell Imaging

Understanding of nanoparticle-bio-interactions within living cells requires knowledge about the dynamic behavior of nanomaterials during their cellular uptake, intracellular traffic and mutual reactions with cell organelles. Here, we introduce a protocol of combined kinetic imaging techniques that enables investigation of exemplary fluorochrome-labelled nanoparticles concerning their intracellu...

Live-cell Imaging Using Fluorescence Microscopy

The ever increasing abundance of different fluorescent proteins and vital dyes that are available to researchers today make it possible to study a large variety of dynamic biological processes in live-cells [1, 2]. Many proteins of interest can be studied by expressing fluorescent fusion proteins in cells, either transiently by transfection or viral infection or permanently using transgenic ani...

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

Conventional imaging techniques can provide detailed information about cellular processes. However, this information is based on static images in an otherwise dynamic system, and successive phases are easily overlooked or misinterpreted. Live-cell imaging and time-lapse microscopy, in which living cells can be followed for hours or even days in a more or less continuous fashion, are therefore v...